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Genetics Outline 2
Topic - Genetics
10.1 Meiosis
10.1.1 Homologous Chromosomes
 chromosomes w/same gene locii in same sequence
 capable of pairing up to form bivalents during meiosis (first phase)
10.1.2 Phases of Meiosis
 exact same as from section 3.3 but reproduced here
 just like in Mitosis, most action happens in prophase
Prophase I
 nuclear membrane disintegrates, etc.
 main thing is that homologous chromosomes line up and come together
 homologous chromosomes: 2 pairs of sister chromatids, not identical but similar
 called tetrad, when joined in tetrad get crossing over (chiasmata/synapsis)
Metaphase I
 nothing splits, but homologous pairs are split and sent to separate ends
Anaphase I
 homologous chromosomes sent to separate ends, begin cytokinesis
Telophase I
 get two new cells, homologous chromosomes in own cell
Prophase II
 break down nuclear envelope, get replication of chromosomes (2n)
Metaphase II
 all genetic info is lined up on metaphase plate in random order
Anaphase II
 get actual split of sister chromatids, chromosomes go to either end of cell
Telophase II
 get reformation of nuclear envelope
 have 4 new cells (not identical to one another) w/n chromosomes
10.1.3 Crossing-over
 very important in variation
 new combinations of genes are formed through this
 synapsis occurs in Prophase I, homologous chromosomes are close together
 this is when two nonsister chromatids overlap, break at chiasmata and re-attach to other chromatid
 the new combinations are called recombinants
10.1.4 Chiasma
 points where homologous chromosomes remain in contact as chromatids move apart during Prophase I
 cross-shaped structure formed by crossing over between chromosomes or two chromatids
 have exchanged genetic material through crossing-over
10.1.5 Genetic Variety
 meiosis results in infinite genetic variety in gametes
 get this through crossing-over in Prophase I and independent assortment in Metaphase I
 results in about 63 trillion combinations for humans (8 million x 8 million = sperm x egg)
 number of different gametes produced is 2 n where n is haploid number
 get 8 million from each gamete cuz 2 23 (23 pairs of homologous chromosomes)
 also added to this variety is crossing-over
10.1.6 Recombination
 reassortment of genes into different combinations from parents
 production of offspring w/new combinations of traits inherited from 2 parents
10.1.7 Mendel’s Law
 2nd law: Law of Independent Assortment
 any one of a pair of characteristics may combine w/either one of another pair
10.1.8 Mendel’s Laws and Meiosis
 Mendel’s 2 nd law applies to traits carried on different chromosomes
 in meiosis, Metaphase I to be exact, any combination of chromosomes is possible
 therefore, any one of a pair of characteristics may combine w/either one of another pair
 also, 1 st law applies (law of segregation) cuz in meiosis sort alleles into separate gametes
10.2 Dihybrid Crosses
10.2.1 Calculate/Predict Genotypes and Phenotypes
 get something like AaBb vs. AaBb
 that’s the P groups (parents)
 then have to determine F 1 genotypes and mention ratios of phenotypes (9:3:3:1)
10.2.2 Identify Recombinants
 recombination: reassortment of genes or characteristics into different combinations from parents
 mostly applies to linked genes, but also to non-linked
 example: cross tall white (Ttrr) w/short red (ttRr)
 F 1 is: tall white (Ttrr), short red (ttRr), tall red (TtRr) and short white (ttrr)
 tall red and short white are recombinants
10.3 Autosomal Gene Linkage and Gene Mapping
10.3.1 Autosomes vs. Sex Chromosomes
 autosomes: all chromosomes that aren’t sex chromosomes
 sex chromosomes: chromosomes that help determine sex of individual
10.3.2 Exchange of Alleles
 crossing-over in Prophase I between non-sister chromatids of homologous pairs can result in variation and exchange of alleles
 pretty much identical to that said in 10.1.3
10.3.3 Linkage Group
 group of genes whose locii are on same chromosome
10.3.4 Example of Linked Genes
 Drosophila: possess 8 chromosomes and easy to breed
 result in offspring didn’t always correspond to predicted values
 only reason is that the two traits tested for are found on same chromosome, thus linked
10.3.5 Determine the Recombinants in Linked Gene Crosses
 read up from 10.2.2
 w/respect to linked genes, the recombinants are the combinations of genes which parent’s didn’t possess
10.3.6 Cross-Over Value
 there will always be 1 copy of parental cuz only 1 chromatid does crossing over
 when show one chromosome crossing, the other one isn’t
 occurrence of crossing-over is proportional to distance between linked genes
 also proportional to number of recmbinants produced
 the close the traits are, the less chance they have for crossing-over & changing material
 the further they are, the more chance they have of crossing-over & changing material
 if linked traits, then get about even numbers in ratios of offspring (1:1 and 1:1)
 cross-over value (COV) = number of recombinants / total offspring x100
 genetic map is linear sequence of genes along chromosome
 not an accurate picture of chromosome, but can give general idea
 max recombination frequency is 50%
 this means that the genes are so far apart on chromosome that crossing-over occurs so frequently it’s as if they were on different chromosomes
 but they’re still linked!
10.3.7 Centimorgan
 units used to express distance between 2 genes on the same chromosome
10.4 Statistical Analysis
10.4.1 Chi-Squared Test
 tells you when the value is ok and when it’s extraneous
 useful in testing a hypothesis, compare actual (observed) results to expected results
 X 2 = S (observed – expected) 2 / expected
 NULL hypothesis: any deviation from the expected is due to chance
10.5 Polygenic Inheritance
10.5.1 Definition
 polygenic: many genes affecting a particular trait
 an additive effect of 2 or more genes on the phenotype (skin colour in humans)
10.5.2 Continuous Variation
 presence of each gene results in mixed output
 get whole range of possibilities
 look at skin colour: AABBCC = very dark skin, aabbcc = very light skin
 any other combination of the 3 genes (this is simplified of course) result in different variations
 if graph phenotypes, get bell-shaped curve, this demonstrates continuous variation
 there are no breaks, get continuous shape
10.5.3 Dihybrid Crosses
 epistasis: standing on top of
 genes whose presence causes other genes to be effective/turned on (control other genes)
 in order for gene A or a to work, gene B must be present
 therefore get different ratio in offspring production
 it means they’re unlinked genes, and that epistasis has occurred
10.6 Applications of Genetics to Agriculture and Horticulture
10.6.1 Inbreeding
 mating between closely related individuals
 most extreme case is self-fertilization in plants
 increase homozygous recessive traits’ occurrence in offspring when inbreed
 don’t get variations
 in human populations get same effect on offspring
 have greater chance of getting recessive genes, but not always the case
10.6.2 Outbreeding
 opposite of inbreeding, mating between non-related individuals
10.6.3 Interspecific Hybridization
 mating between different species
 produce offspring, but offspring is infertile
 horse + donkey = mule (sterile)
 species: 2 animals are of same species if can mate and produce viable offspring
10.6.4 Polyploidy
 extra sets of chromosomes
 3n = triploidy; 4n = tetraploidy
 happens in plants, growing wheat, rice, rye, barley, maize, etc.
 non-disjunction occurs
 tends to produce hardy/strong individuals
10.6.5 F1 Hybrid Vigour
 cross-bredding between 2 different varieties
 offspring more vigourous than parentals
 elminates homozygous recessive especially if trait is deleterious (bad, detrimental)
 heterozygous advantage
10.6.6 Examples
 inbreeding: self-fertilization in plants
 used in breeding sheep, cattle and pigs to strengthen desirable characteristics
 outbreeding: done between breeding lines w/in species or between species
 w/in species: short-horned cattle vs. Black Angus cattle = tasty beef w/rapid growth
 between species: horse + donkey = mule (sterile), stronger and more resistant to disease
 also have Macoun apple + crab apple = Liberty apple
 similar in taste to Macoun but more resistant to disease
 polyploidy: strawberries, Daylilies, Freesias, many plants bought for flowers
10.6.7 3 Examples of Transgenic Techniques
insulin gene: humans -> bacteria
 now make insulin for nothing cuz take out gene
 put gene into bacteria’s DNA and BLAMMO!
frost resistance: flounder -> tomatoes
 took gene from fish, put it into tomatoes
blood clotting (factor IX): humans -> sheep (milk)
increases production
first ever example
firefly gene (light): firefly -> tobacco plant
10.6.8 Ethical problems
 biodiversity, just cuz we can should we?
 do we have the right?
 creation of new species/improve on species? do we have right?
 humans? or not? human worth? unnatural?
 super plants? resistant to herbicides/pesticides
 competing w/other species? difficult to control
 the bacteria can become pathogenic (super bacteria)
 who profits from this? -> Monsanto pattented Canola
 patents on transgenic organisms
 biological warfare – newly engineered bacteria
 DNA in foods (Genetically Modified Organisms)
10.6.9 Biodiversity
 growing human population needs it, now we rely on 3 plants (rice, corn, wheat)
 could get problem like potato famine in Ireland
 research being done to see if can use other wild plants to become new crop plants
 also to improve resistance to insects/disease
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