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Cell Outline
Topic - Cells 
7.1 Membranes 
 membrane is a collage of different proteins embedded in fluid matrix of lipid bilayer
 proteins determine most of membrane’s specific functions
 two major types: integral and peripheral
 integral go far enough into membrane for hydrophobic regions to be surrounded by hydrocarbon tails of membrane
 some reach only partway across membrane, some completely span it
 these have hydrophobic regions surrounded by hydrophilic regions that are outside membrane
 peripheral ones aren’t embedded in membrane at all
 append to surface often on exposed parts of integral proteins
 inside cell, peripheral and integral proteins can be held in place by cytoskeleton matrix
 also, fibres of the cytoskeleton adhere to specific membrane proteins
 cell-cell recognition is vital to functionality of organsim
 important in cell specialization
 also basis of rejection of other cells (antibody recognition)
 oligosaccharides (smaller than polysaccharides) are covalently bonded to lipids in membrane
 some form molecules called glycolipids (glyco = presence of carbos)
 most form bonds w/proteins, thus called glycoproteins
7.1.1 Relationship 
 relationship between nucleus, RER, Golgi and cell surface membrane is in protein synthesis
 begins in nucleus (production of mRNA), mRNA attaches to ribosome in cytoplasm bound to ER
 translates genetic material into beginning structure of protein
 as polypeptide chain grows, flows through ER into cisternal space through pores
 folds into specific shape, and if made for exocytosis, it’s transferred to specific section of ER
 fuses w/transport vesicle for transport to golgi
 protein enters golgi, enzymes shape/modify protein to final product, move through apparatus
 then fuses w/vesicle again, sent to plasma membrane, once there it’s secreted out
 structure of cell surface membrane similar to that of nuclear membrane, ER and Golgi
 possible to exchange membrane sections between each
 nucleus contains all the genetic info for the creation of proteins
 RER has ribosomes needed to make protein, absorbed by lumen of RER, then “pinched off” through cell surface membrane
7.1.2 Transport 
 vesicles transport through cell membranes via transport proteins
 exocytosis is secretion of macromolecules by fusing vesicles w/plasma membrane
 vesicle usually originates in the Golgi or the ER
 endocytosis is formation of a vesicle around macromolecule
 vesicle originates from membrane
 3 types of endocytosis: phagocytosis, pinocytosis, and receptor-mediated endocytosis
 phagocytosis: when cell engulfs particle by wrapping pseudopodia around it, packs it w/membrane-enclosed sac
 pinocytosis: cell gulps droplets of extracellular fluid in tiny vesicles; very unspecific in the substances transported while receptor-mediated endocytosis is very specific.
7.1.3 Membrane Proteins
 2 kinds of membrane proteins
 integral protein embedded in lipid layer. Most span all the way across the membrane, but all do not
 peripheral proteins are proteins that are attached to the membrane on the cytoplasm side of the membrane
 usually attached to the exposed end of integral proteins
 sometimes held in place by filaments in the cytoskeleton
 can be outside or inside the membrane, all depends on polarity of protein
 polar protein (hydrophilic) is embedded between membrane phosphate heads (extrinsic, peripheral) on outside of cell; usually attached to hydrophilic parts of integral proteins
 nonpolar proteins (hydrophobic) are inside the membrane (intrinsic, integral)
 some proteins that have both regions situate themselves so that hydrophilic part meets the phosphate heads and the hydrophobic part lies in the centre of the membrane
 proteins can serve as “anchors”
 shape of cell is determined in part by organization of cytoskeleton
 the microfilaments that make it up are connected to specific proteins in the membrane that help define the cell’s shape
 also, the way in which the protein is attached to the cytoskeleton defines the membrane’s shape
 some membrane proteins are used in active transport or the transport of specific elements
 called transport proteins
 have pores that only fit shape of specific element they are made for, thus only allowing them to enter/exit the cell
7.1.4 Functions
 I don’t know
7.2 Cell Division – Mitosis
7.2.1 Chromosomes
 mitosis, increase number of cells w/out changing genetic materials
 4 phases: prophase, metaphase, anaphase and telophase
 interphase: DNA replicates
 prophase: chromosomes are visible (coiling), centrioles move to opposite ends of cell
 spindle formation, nucleolus invisible, nuclear membrane disintegrates
 metaphase: chromosomes move to “equator”, metaphase plate, centromeres attach to spindle
 anaphase: chromatids separate and move to opposite ends
 telophase: spindle disappears, centrioles replicate, nuclear membrane appears, chromosomes unwind
7.2.2 Animal vs. Plant
 process summarised as:
 dispersing of nuclear material
 movement of centrosomes to opposite ends of cell, microtubules develop into spindle
 supercoiling of chromatin, attachment to spindle fibres, separation and movement of chromatids
 animal cells have centrioles which form focus of spindle, plants don’t but still form spindle
 cytokinesis occurs in animals by pinching in of cell surface membrane
 microfilaments formed around cell’s equator begin to constrict
 in plant cells, cell plate formed by vesicles from Golgi moving to equator and merging
7.3 Differentiations and Functional Specialization of Cells
7.3.1 Functions of Life
 prokaryotes carry out all functions of life (reproduction, respiration, etc.)
7.3.2 Specialized Functions
 cells in eukaryotes differentiate to carry out specific functions
7.3.3 Tissue
 group of similar cells performing specific task
 blood cells, epithelial cells, etc.
7.3.4 Organ
 structural unit made of group of tissues which work together to perform function
 heart, liver, etc.
7.3.5 Organ System
 several organs working together to carry out specific function
 digestive system, circulatory system
7.3.6 Relationship
 cells > tissues > organs > organ system
 each higher structure is made up of the lower ones
7.3.7 Calculate Magnification
 Magnification = Diagram Size / Actual Size
7.3.8 Specialization of Cells
 all cells contain all genetic information needed to make any cell throughout the body
 only specific cells activate specific regions of DNA
 thus they specialize by expression of some of their genes while others aren’t
7.3.9 Differentiation
 differentiation of cell determined by its relative position to others & chemical gradients
 all cells originate from zygote
 determination of specialization can involve inheritance of different molecules or interactions between neighbouring cells
 presence of certain cell type causes neigbouring cell to differentiate in specific way
 called induction
 occurs because one cell releases substance which diffuse to other cell, cause differentiation (theory)
 evidence contradictory, conclusions can’t be drawn
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