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Cell Biology Outline
 Topic - Cells
1.1 Cell Theory
1.1.1 Contributions
Robert Hooke: studied cork, names structures “cells”; 1665
Anton van Leeuwenhoek: discovered unicellular organisms; 1675
Matthias Schleiden: “all plants are made of cells”; 1838
Theodore Schwann: “all animals also made of cells”; 1839
Rudolph Virchow: “all cells come from cells”; 1855
1.1.2 Advantages of Light Microscope
 1,000X magnification
 specimen alive
 colour
 light
 resolution 0.2 um
1.1.3 Advantages of Electron Microscope
 1,000,000X magnification
 specimen dead
 electrons
 resolution 0.2 nm
 3D image
1.1.4 Organelle
 one of several formed bodies with specialized function
 suspended in cytoplasm
 found in eukaryotes
1.1.5 Size
 molecules 1 nm
 membrane thickness 10 nm
 virus 100 nm
 bacteria 1 um
 organelle up to 10 um
 cells up to 100 um
 cells are so big due to their 3D shape compared to the other stuffs
 animal cells generally smaller than plant cells
 yolk of egg is one cell
1.1.6 Surface Area vs. Volume
 if cell too large, diffusion distance too long to be efficient, SA:V ratio too small to allow change
 V increase quicker than SA, eventually creates problem for cell
 combat this by increasing SA: protruding extensions from cell, flattening of cell
1.2 Prokaryotic Cell Structure
3 outside layers
capsule (slime capsule/layer)
 very unique organization of matter, main ingredient is peptidoglycan molcules
 makes it very strong, resistant to infiltrations
 use different amino acids than what proteins use, impenetrable to certain things
cell wall (esentially same as plant)
plasma membrane
 very well protected, no true nucleus but has big chunk of DNA
 has plasmids; extra DNA that most bacteria have, usually circular
 carries genetic info & divides/replicates as any other
 use this one most of the time in gene-splicing and genetic engineering
1.2.1 Draw a regular prokaryotic cell
1.2.2 Functions
 ribosome: synthesize proteins using info from mRNA
 mesosome: pocket of cell surface membrane; possible function in cellular resp.
 slime capsule: outside cell wall, made of polysaccharide or polypeptide; allows cell to stick to surfaces (soil, rocks, cells, teeth, etc.)
 cell wall: made of amino sugars; protects cell, gives shape
 flagellum: long, thin thread rotated to propel cell
 cell surface membrane: separates cell from enviro, maintains homeostasis
 plasmid: circular DNA in bacteria
 naked nucleic acid: control centre of cell
 cytoplasm: few organelles and enzymes
 photosynthetic membranes: possess pigments for photophosphorilation
1.3 Eukaryotic Cell Structure
1.3.1 Origin, Theory of Endosymbiosis
fossil record dates back 1.5 billion years endosymbiont theory: mitochondria and chloroplasts originally independent prokaryotes; developed in close relationship w/other prokaryote until became one
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Prokaryotes, Chloroplasts and Mitochondria
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Eukaryotes
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DNA
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 circular
 not contained in chromosomes
 not contained in nucleus
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 linear
 contained in chromosomes
 contained in nucleus
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Ribosomes
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70S
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80S
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Size
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0.5-5 um
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20 um
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1.3.2 Draw generalised animal cell
1.3.3 Functions
 nucleus: largest cell organelle, contains chromatin; controls activities of cell
 ribosome: made of protein and rRNA attached to RER or in cytoplasm
 RER: system of membranes continuous w/nuclear envelope; site for protein synthesis
 lysosome: contains digestive enzymes; intracellular digestion, “suicide bag”
 Golgi: system of membranes for intracellular transport; formation of vesicles for exocytosis
 mitochondrion: double membrane around matrix; site for Krebs cycle/electron transport chain/oxidative phosphorylation
1.3.4/1.3.5 Similarities and Differences
Feature
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Similarities
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Differences
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Prokaryotes
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Eukaryotes
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form
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cells
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unicellular
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multicellular
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genetic material
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DNA
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Circular
no chromosomes
no nucleus
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linear
chromosomes
nucleus
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protein synthesis
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ribosomes
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70S
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80S
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flagellum
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possible
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simple
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complex
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cell wall
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possible
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polysaccharides
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cellulose
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respiration
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process
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mesosomes
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mitochondria
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photosynthesis
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process
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membranes
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chloroplasts
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1.3.6
Feature
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Plant cell
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Animal cell
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cell wall
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present
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absent
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chloroplasts
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present
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absent
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vacuole
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large, permanent
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small, temporary
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reserve food
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starch
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glycogen
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1.3.7 Cell Wall
 main function is to provide mechanical support for cell
 plays important roll in turgor, needs to be firm because of lack of skeleton (as in animals)
 plant cells are hypertonic to enviro, take water by osmosis; cell swells and pushes against wall
 animal cell would burst, but cell wall strong enough to hold it together
 does this until water taken into cell equals that lost, then said to be turgid
 allows movement of water and mineral salts across & laterally
 made up of microfibrils; polysaccharide molecules, form long straight chains w/few covalent bonds; helps create their strength
 good for products like paper and cotton
1.4 Membranes
1.4.1 Draw diagram of membrane
 fluid mosaic model proposed in 1972 by Singer and Nicolson
 consists of phospholipid bilayer w/proteins throughout lipid molecules in between lipids have hydrophobic cholesterol molecules; if have lots of cholesterol, membrane less fluid
1.4.2 Hydrophobic/Hydrophilic Properties
 considered to be amphipatic
 phosphate group polar (hydrophilic); this part reacts w/water and found on surface of membrane, outside and inside
 non-polar regions are hydrophobic tails, inside molecule to avoid contact w/water
 some mebranes have carbohydrate group attached to phosphate group, then call glycolipid
 some proteins have same deal, called glycoprotein and function as receptors for hormones, etc.
 integral proteins embedded in membrane, usually hydrophilic w/hydrophobic midsection
 peripheral ones are found just inside membrane, hydrophilic and don’t interact w/hydrophobic
 core proteins in membrane can be structural and/or functional can anchor cell, act as carriers, facilitate transport, electron carriers, receptors, etc.
1.4.3 Diffusion
 the movement of particles from an area of high concentration to area of low concentration down gradient
 solute is stuff being dissolved
 solvent is medium in which solute dissolved
1.4.4 Osmosis
 passive movement of water molecules from low solute concentration to high solute concentration
 across partially permeable membrane
 structures can be virtually impermeable, partially or totally permeable
 partially permeable ones have certain structure to allow some molecules to pass through while other can’t
1.4.5 Passive Transport
 transport proteins, some require energy & some don’t
 when molecules flow through freely, it’s passive transport
 cell surface membrane keeps cell content together, maintains homeostasis (diffusion)
 when substances are small, carry little/no electrical charge, then pass through freely
 passive transport doesn’t need energy, move from high to low concentration
 facilitated diffusion, the use of hydrophilic channels (transport proteins) to allow hydrophilic things to pass by membrane
 doesn’t need energy, from high to low concentration
1.4.6 Active Transport
 carrier-assisted transport, membrane loaded with transport proteins
 makes cell highly selective in what it accepts
 some cells need to take things that are already in high concentration, use carrier proteins
 “sodium-potassium pump”
 protein in membrane moves sodium out of cell and potassium into cell, sodium is high outside but still moves it out
 diffusion across gradient, energy needed (ATP), create potential difference
*A solution that is hypertonic will also have a higher Osmotic Pressure
1.4.7 Endocytosis vs. Exocytosis
 endocytosis: cell takes up substance by surrounding it w/membrane molecules
 pinocytosis (fluids) called “cell drinking” and phagocytosis (solids) called “cell eating”
 exocytosis: reverse of endocytosis, cell secretes molecules by fusing vesicles w/plasma membrane
 vesicle from golgi moved to membrane, when come into contact lipids in membrane rearrange and fuse
 become continuous, contents of vesicle spill to outside of cell
1.5 Mitosis
 cell division enables multicellular organism to grow/develop from single cell
 also aids in repair and renewal, replacing old cells (i.e. bone marrow and blood cells)
 involves distribution of DNA to daughter cells
 all DNA in cell is called genome
 compact folding/coiling of DNA form chromosomes
 cell preparing to divide copies genes, allocates them equally, then separates (mitosis)
Eukaryotic cell:
Two phases, interphase and M phase
 the end result of mitosis is 2 identical cells (to each other & to original they came from in terms of DNA chromosomes)
 before mitosis starts, each of the chromosomes are duplicated
 in humans its 92 (S phase)
 sister chromatids, 46 of these
 centrioles moving to opposite sides of cell, nuclear membrane breaking down
 DNA in chromosomes (sister chromatids) can be seen
 spindles attach to chromatids, bring centromere to metaphase plate
interphase
 3 steps: G1, S, G2 (G for growth, S for synthesis)
 G1, typically longest phase, most proteins made during this phase
 S, cell replicates DNA in nucleus for cell division, exact copy of all DNA (only 1 copy)
 G2, cell may continue to grow & produces number of molecules
 signals initiation of mitosis
 M phase, actual division of cells (follows in next steps)
prophase
 where most action happens, chromatin -> chromosomes
 making of tubules/centrioles, etc.
metaphase
 sister chromatids line up on metaphase plate ready to be split
anaphase
 chromatids are separated, can call them true chromosomes (46 on each side)
telophase
 starts to produce nuclear membrane around chromosomes
 cytokinesis is where start to get plate, division of cytoplasm
 now have 2 identical cells (DNA wise) and are in interphase again
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